CD3 epsilon antibody

Catalog No. ABIN6941031

This Mouse Monoclonal antibody specifically detects CD3 epsilon in FACS, IHC, IF and Func. It exhibits reactivity toward Human.

Quick Overview for CD3 epsilon antibody (ABIN6941031)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Clone: OKT3

Product Details anti-CD3 epsilon Antibody

Purpose: CD3e (T-Cell Marker) Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

Purification: 200ug/ml of Ab purified from rabbit anti-serum by Protein A.

Immunogen: Human PBMC s

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Jurkat cells. Tonsil and lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (acetone fixed frozen sections), Immunofluorescence (1-2 μg/mL), Functional Studies (Order Ab without Azide), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

CD3 epsilon, CD3 TCR complex, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Jurkat cells or PBMCs. Tonsil and lymph node

Molecular Weight: 23kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway