CD5 antibody

Catalog No. ABIN6941053

The Mouse Monoclonal anti-CD5 antibody is suitable to detect CD5 in samples from Human, Rat, Cow and Rabbit. It has been validated for FACS, IHC and IF.

Quick Overview for CD5 antibody (ABIN6941053)

Target: CD5

Reactivity: Human, Rat, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C5-473-CD5-54-F6

Product Details anti-CD5 Antibody

Purpose: CD5 (Mantle Cell Lymphoma Marker) Antibody

Specificity: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human CD5 recombinant protein (C5/473), A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Known Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background:

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

293T, Ramos or MOLT-4 cells. Tonsil.

Molecular Weight: ~67kDa

Gene ID: 921, 58685

UniProt: P06127