CD6 antibody

Catalog No. ABIN6941062

The Mouse Monoclonal anti-CD6 antibody (Clone SPV-L14) (ABIN6941062) specifically detects CD6 in FACS, WB and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for CD6 antibody (ABIN6941062)

Target: CD6

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: SPV-L14

Product Details anti-CD6 Antibody

Purpose: CD6 (Negative Marker of T-regulatory Cells) Antibody

Specificity: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: CD8+ cytotoxic T-cell clone

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: CCRF-CEM, Jurkat cells, Tonsil.

Known Application: Flow Cytometry (1-2 μg/mL), ,Immunofluorescence (0.5-1 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD6

Target: CD6

Alternative Name: CD6

Background:

T12, TP120

CCRF-CEM, Jurkat cells, Tonsil. Human Spleen. Raji. MCF7. PBMC.

Molecular Weight: 90-130kDa

Gene ID: 923, 744366

UniProt: P30203