CD8 alpha antibody
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- Target See all CD8 alpha (CD8A) Antibodies
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD8 alpha antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. This MAb binds to the CD8 alpha chain, at an epitope distinct from that recognized by UCHT14.
- Purification
- Purified by Protein A/G
- Immunogen
- Thymocytes followed by Sezary T cells.
- Clone
- UCHT4
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD8A Primary Antibody
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- Application Notes
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Positive Control: Jurkat, HuT78 or hPBL. Tonsil.
Known Application: Flow Cytometry (1-2 μg/million cells), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- Alternative Name
- CD8A (CD8A Products)
- Synonyms
- cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody
- Molecular Weight
- 32kDa
- Gene ID
- 925
- UniProt
- P01732
- Pathways
- TCR Signaling
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