Recombinant CD74 antibody

Catalog No. ABIN6941259

The Rabbit Monoclonal anti-CD74 antibody is suitable to detect CD74 in samples from Human, Mouse and Baboon. It has been validated for FACS, WB, IHC and StM.

Quick Overview for Recombinant CD74 antibody (ABIN6941259)

Target: CD74 (HLA-DR-gamma (CD74))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Baboon

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CLIP-3127R

Product Details anti-CD74 Antibody

Specificity: It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

No Cross-Reactivity: Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: SU-DHL-4 lymphoma cells

Isotype: IgG

Application Details

Application Notes:

Positive Control: 293T, Daudi or Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Molecular Weight: 33-41kDa

Gene ID: 972

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints