CDK1 antibody (C-Term)

Catalog No. ABIN6941282

This anti-CDK1 antibody is a Mouse Monoclonal antibody detecting CDK1 in WB, IHC, IF, IP, Act, KAA and StM. Suitable for Human, Mouse, Rat, Chicken, Xenopus laevis, Guinea Pig and Marmota monax.

Quick Overview for CDK1 antibody (C-Term) (ABIN6941282)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis, Guinea Pig, Marmota monax

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Activation (Act), Kinase Activity Assay (KAA), Staining Methods (StM)

Clone: A17-1-1

Product Details anti-CDK1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes a 34 kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Purification: Purified by Protein A/G

Immunogen: C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Human tonsil (IHC).

Known Application: Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence (1-2 μg/mL), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDC2

Molecular Weight: 34kDa

Gene ID: 983

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA