CD3 epsilon antibody

Catalog No. ABIN6941302

The Armenian Hamster Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Mouse. It has been validated for FACS, IF and Func.

Quick Overview for CD3 epsilon antibody (ABIN6941302)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody

Purpose: CD3e, Mouse Antibody

Specificity: CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful forin vitroblocking and activation assays, as well as apoptosis induction andin vivoT cell depletion.

No Cross-Reactivity: Human

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: H2Kb specific Mouse cytotoxic T lymphocyte clone BM10 37

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: MPBMC's or Thymus or Lymph Node.

Known Application: Functional Studies (Order Antibody without BSA & Azide),Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: Cd3e

Background:

CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); IMD18; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE

MPBMC's or Thymus or Lymph Node.

Molecular Weight: 23kDa

Gene ID: 12501, 210361

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway