CLEC9A antibody

Catalog No. ABIN6941306

The Rat Monoclonal anti-CLEC9A antibody is suitable to detect CLEC9A in samples from Mouse. It has been validated for FACS, WB and IF.

Quick Overview for CLEC9A antibody (ABIN6941306)

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CLEC9A antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: 1F6

Product Details anti-CLEC9A Antibody

Purpose: CLEC9A Antibody

Specificity: This Mab recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

Isotype: IgG1

Application Details

Application Notes:

Positive Control: Mouse spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL),Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CLEC9A

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Alternative Name: CLEC9A

Background:

C type lectin domain family 9, member A, C-type lectin domain family 8 member A, CLEC9A, DNGR1, HEEE9341, PRO34046, UNQ9341, CD370

Mouse spleen.

Molecular Weight: 27kDa