Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody

Catalog No. ABIN6941352

The Mouse Monoclonal anti-Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody (Clone M2-7C10-M2-9E3-T311-HMB45) (ABIN6941352) specifically detects Melanoma Marker (MART-1 + Tyrosinase + Gp100) in IHC, ISt and StM. The antibody is reactive with Human samples.

Quick Overview for Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody (ABIN6941352)

Target: Melanoma Marker (MART-1 + Tyrosinase + Gp100)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: M2-7C10-M2-9E3-T311-HMB45

Product Details

Specificity: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Purification: Purified by Protein A/G

Immunogen: Recombinant hMART-1 protein (M2-7C10, M2-9E3), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Melanoma Marker (MART-1 + Tyrosinase + Gp100)

Molecular Weight: 20-22kDa (doublet) (MART), 70-80kDa (Tyrosinase), 90-100kDa (gp100)

Gene ID: 2315, 7299, 6490

UniProt: Q16655, P14679, P40967