Keratin 77 antibody

Catalog No. ABIN6941447

The Mouse Monoclonal anti-Keratin 77 antibody has been validated for IHC and StM. It is suitable to detect Keratin 77 in samples from Human and Rat.

Quick Overview for Keratin 77 antibody (ABIN6941447)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: KRTL-1077

Product Details anti-Keratin 77 Antibody

Specificity: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Cross-Reactivity (Details): Shows broad species reactivity.

Purification: Purified by Protein A/G

Immunogen: Recombinant human KRT77 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Skin, Squamous cell carcinoma (SCC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77

Molecular Weight: 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

Gene ID: 374454

UniProt: Q7Z794