Actin antibody (AA 3-100)

Catalog No. ABIN6942039

This Rabbit Polyclonal antibody specifically detects Actin in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Actin antibody (AA 3-100) (ABIN6942039)

Target: Actin

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Actin Antibody

Binding Specificity: AA 3-100

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ACTA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Actin

Target: Actin

Background:

Synonyms: muscle Actin, ACTS_HUMAN, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, Actin alpha skeletal muscle, actin, alpha 1, skeletal muscle 1, actin, alpha 1, skeletal muscle, actina, aktin, alpha Actin 1, alpha skeletal muscle Actin, alpha skeletal muscle, alpha-actin, Beta cytoskeletal actin, nemaline myopathy type 3.

Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Gene ID: 58

UniProt: P68133