Angiopoietin 1 antibody

Catalog No. ABIN6942099

This Rabbit Monoclonal antibody specifically detects Angiopoietin 1 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for Angiopoietin 1 antibody (ABIN6942099)

Target: Angiopoietin 1 (ANGPT1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7B11

Product Details anti-Angiopoietin 1 Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by Protein A.

Immunogen: Human Angiopoietin 1 between 250-350 amino acids

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: Angiopoietin 1

Background:

Synonyms: Angiopoietin-1, ANG-1, ANGPT1

Background: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Gene ID: 284

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process