APAF1 antibody

Catalog No. ABIN6942119

The Rabbit Monoclonal anti-APAF1 antibody (Clone 4G2) (ABIN6942119) specifically detects APAF1 in WB, IHC, IHC (p) and IF (cc). The antibody is reactive with Human and Mouse samples.

Quick Overview for APAF1 antibody (ABIN6942119)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4G2

Product Details anti-APAF1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant human Apaf-1 between 1 to 300 amino acids

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1

Background:

Synonyms: Apoptotic protease-activating factor 1, APAF-1, APAF1

Background: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity