ATG5 antibody

Catalog No. ABIN6942172

The Rabbit Monoclonal anti-ATG5 antibody has been validated for WB, IHC and IF (cc). It is suitable to detect ATG5 in samples from Human, Mouse and Rat.

Quick Overview for ATG5 antibody (ABIN6942172)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4G5

Product Details anti-ATG5 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human ATG5 between 1 to 200 amino acids

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background:

Synonyms: Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5

Background: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT2 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy