CASP14 antibody

Catalog No. ABIN6942355

Product Details anti-CASP14 Antibody

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CASP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human Caspase-14 aa 100-200

Clone: 8C4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CASP14

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: Caspase-14 (CASP14 Products)

Synonyms: CASP14 antibody, MICE antibody, mini-ICE antibody, caspase 14, apoptosis-related cysteine peptidase antibody, caspase 14 antibody, caspase-14-like antibody, caspase-14 antibody, CASP14 antibody, Casp14 antibody, LOC485601 antibody, LOC780455 antibody

Background:

Synonyms: Caspase-14, CASP-14, CASP14

Background: Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).

Gene ID: 23581

UniProt: P31944