DNMT3A antibody

Catalog No. ABIN6943093

The Rabbit Polyclonal anti-DNMT3A antibody has been validated for WB, ELISA, IP and ChIP-seq. It is suitable to detect DNMT3A in samples from Human.

Quick Overview for DNMT3A antibody (ABIN6943093)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-DNMT3A Antibody

Cross-Reactivity: Human

Purification: Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Immunogen: Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IP(1-2 μg)
ChIP-seq()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 μg/μL

Buffer: PBS with 0.05 % sodium azide and 0.05 % Proclin300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background:

Synonyms: DNMT3A2, TBRS

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

Gene ID: 1788

UniProt: Q9Y6K1