H2AFY antibody (AA 150-300)

Catalog No. ABIN6943601

The Rabbit Monoclonal anti-H2AFY antibody (Clone 6C8) (ABIN6943601) specifically detects H2AFY in WB, IHC (p), IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for H2AFY antibody (AA 150-300) (ABIN6943601)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 6C8

Product Details anti-H2AFY Antibody

Binding Specificity: AA 150-300

Purpose: H2AFY (6C8) Monoclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human macroH2A.1 aa 150-300

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY

Background:

Synonyms: macroH2A.1, Core histone macro-H2A.1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, H2A histone family member Y.

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2AFY is involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. H2AFY inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

Gene ID: 9555

UniProt: O75367