H2AFY antibody

Catalog No. ABIN6943601

Product Details anti-H2AFY Antibody

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human macroH2A.1 aa 150-300

Clone: 6C8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY (H2AFY Products)

Synonyms: H2A.y antibody, H2A/y antibody, H2AF12M antibody, H2AFJ antibody, MACROH2A1.1 antibody, mH2A1 antibody, macroH2A1.2 antibody, mH2a1 antibody, macroH2A1 antibody, wu:fa93c12 antibody, zgc:136891 antibody, h2a.y antibody, h2a/y antibody, h2af12m antibody, h2afj antibody, macroh2a antibody, mh2a1 antibody, core histone macro-H2A.1 antibody, H2A histone family, member Y antibody, H2A histone family member Y antibody, H2A histone family member Y L homeolog antibody, LOC100230612 antibody, H2AFY antibody, LOC481514 antibody, H2afy antibody, h2afy antibody, h2afy.L antibody

Background:

Synonyms: macroH2A.1, Core histone macro-H2A.1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, H2A histone family member Y.

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2AFY is involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. H2AFY inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

Gene ID: 9555

UniProt: O75367