His Tag antibody (HRP)

Catalog No. ABIN6943618

The Rabbit Polyclonal anti-His Tag antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect His Tag in samples from Tag.

Quick Overview for His Tag antibody (HRP) (ABIN6943618)

Target: His Tag

Reactivity: Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-His Tag Antibody (HRP)

Cross-Reactivity: Tag

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from His Tag

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background:

Synonyms: HisX6, 6His, His Tag, 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag.

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.