His Tag antibody

Catalog No. ABIN6943619

This anti-His Tag antibody is a Rabbit Polyclonal antibody detecting His Tag in WB, ELISA, ICC, IHC (fro), IF (p), IF (cc) and IHC (p). Suitable for Tag.

Quick Overview for His Tag antibody (ABIN6943619)

Target: His Tag

Reactivity: Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-His Tag Antibody

Cross-Reactivity: Tag

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from His Tag

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background:

Synonyms: HisX6, 6His, His Tag, 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag.

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.