IKK-alpha /IKK-beta antibody

Catalog No. ABIN6943783

The Rabbit Monoclonal anti-IKK-alpha /IKK-beta antibody is suitable to detect IKK-alpha /IKK-beta in samples from Mouse, Human and Rat. It has been validated for WB, IF (cc) and IHC (p).

Quick Overview for IKK-alpha /IKK-beta antibody (ABIN6943783)

Target: IKK-alpha /IKK-beta

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4D4

Product Details anti-IKK-alpha /IKK-beta Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human IKK alpha + IKK beta between 450 amino acids to C-terminus

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKK alpha + IKK beta

Background:

Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, IKK1, NFKBIKA, TCF-16, CHUK, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, IKK2, NFKBIKB, IKBKB

Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

Gene ID: 3551

UniProt: O14920