MEK1/2 antibody

Catalog No. ABIN6944005

The Rabbit Monoclonal anti-MEK1/2 antibody is suitable to detect MEK1/2 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Crucian Carp and Fish. It has been validated for WB, IF (cc), IF (p), IHC (p) and FACS.

Quick Overview for MEK1/2 antibody (ABIN6944005)

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Crucian Carp, Fish

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 4H7

Product Details anti-MEK1/2 Antibody

Specificity: The antibody will detect both MAP2K1 and MAP2K2

Cross-Reactivity: Fish, Human, Mouse, Rat, Zebrafish (Danio rerio)

Cross-Reactivity (Details): Crucian carp (Carassius carassius)

Purification: Purified by Protein A.

Immunogen: Human MAP2K1 + MAP2K2 aa 1 to the C-terminus

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MAP2K1/2

Background:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAP2K1, MEK1, MKK2, PRKMK1, MAPKK1, Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MAP2K2, MEK2, MKK2, PRKMK2, MAPKK2

Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Gene ID: 5604, 5605

UniProt: Q02750, P36507