MDFI antibody (AA 101-200) (Cy5.5)

Catalog No. ABIN6944058

This Rabbit Polyclonal antibody specifically detects MDFI in IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for MDFI antibody (AA 101-200) (Cy5.5) (ABIN6944058)

Target: MDFI (MyoD Family Inhibitor (MDFI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFI antibody is conjugated to Cy5.5

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MDFI Antibody (Cy5.5)

Binding Specificity: AA 101-200

Specificity: In Mice, there is possible cross-reactivity with Glycoprotein endo-alpha-1,2-mannosidase (67 % homology with immunogen sequence, 53 kDa)

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MDFI

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI

Background:

Synonyms: I MF, I mfa, Inhibitor of MyoD family a, Mdfi, MDFI_HUMAN, MyoD family inhibitor, Myogenic repressor I-mf, OTTHUMP00000016375, OTTHUMP00000016376, OTTHUMP00000196103, OTTHUMP00000196414, OTTHUMP00000196415.

Background: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).

Gene ID: 4188

UniProt: Q99750

Pathways: Maintenance of Protein Location