MLKL antibody (pSer345)

Catalog No. ABIN6944093

This anti-MLKL antibody is a Rabbit Monoclonal antibody detecting MLKL in WB, IHC (p) and IF (cc). Suitable for Mouse.

Quick Overview for MLKL antibody (pSer345) (ABIN6944093)

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Reactivity: Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 7G4

Product Details anti-MLKL Antibody

Binding Specificity: pSer345

Cross-Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL

Background:

Synonyms: Phospho-MLKL (S345), pMLKL Ser345, Mixed lineage kinase domain-like protein, Mlkl

Background: Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Gene ID: 74568

UniProt: Q9D2Y4