Recombinant NCK1 antibody (AA 1-200)

Catalog No. ABIN6944225

The Rabbit Monoclonal anti-NCK1 antibody (ABIN6944225) specifically detects NCK1 in WB, FACS, IHC (p), IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant NCK1 antibody (AA 1-200) (ABIN6944225)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NCK1 Antibody

Binding Specificity: AA 1-200

Purpose: NCK1 Recombinant Antibody

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein corresponding human Nck1 aa 1-200

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:200-400), IHC-P(1:50-200), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background:

Synonyms: Cytoplasmic protein NCK1, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha, Nck-1, NCK1, NCK.

Background: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals