NEFL antibody (AA 31-130)

Catalog No. ABIN6944267

This anti-NEFL antibody is a Rabbit Polyclonal antibody detecting NEFL in ELISA, ICC, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Rat and Mouse.

Quick Overview for NEFL antibody (AA 31-130) (ABIN6944267)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NEFL Antibody

Binding Specificity: AA 31-130

Specificity: SNDLKSIRTQEK-Y 80-91/543

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NF-L

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NF-L

Background:

Synonyms: Neurofilament L, Neurofilament 68, Neurofilament triplet L, 70 kD Neurofilament Light, 68 kDa neurofilament protein, CMT 1F, CMT 2E, CMT1F, CMT2E, FLJ53642, Light molecular weight neurofilament protein, NEFL, Neurofilament light, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF L, Neurofilament triplet L protein, NF 68, NF L, NF68, NFL, NFL_HUMAN.

Background: Neurofilament light polypeptide also called NF-L, Neurofilament triplet L protein, 68 kDa neurofilament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Belongs to the intermediate filament family.

Gene ID: 4747

UniProt: P07196