Reticulon 4 antibody

Catalog No. ABIN6944299

The Rabbit Monoclonal anti-Reticulon 4 antibody has been validated for WB, IF (cc), IF (p), IHC (p) and FACS. It is suitable to detect Reticulon 4 in samples from Human and Mouse.

Quick Overview for Reticulon 4 antibody (ABIN6944299)

Target: Reticulon 4 (RTN4)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 7A12

Product Details anti-Reticulon 4 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human Nogo aa 1-200

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: Nogo-B

Background:

Synonyms: Reticulon-4B, RTN 4B, Foocen-M, RTN-xS, Neurite outgrowth inhibitor, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog,

Background: Reticulon-4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. It regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 is also Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Isoform 2 of the Reticulon-4 protein, also known as Nogo-B or Reticulon-4B, reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 (Nogo-B) and isoform 3 (Nogo-C) inhibit BACE1 activity and amyloid precursor protein processing. Nogo-B is mainly localized primarily in the endoplasmic reticulum (ER), where it participates in the maintenance of ER tubular shape and functions. Nogo-B is expressed in most tissues and is associated with diverse processes such as vascular remodeling, liver regeneration, liver cirrhosis, pulmonary hypertension, and inflammatory bowel disease.

Gene ID: 57142

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome