PKD2 antibody (Protein Kinase D2)

Details for Product anti-PKD2 Antibody No. ABIN6944658
Antigen
  • PKD2
  • nPKC-D2
  • AI325941
  • RGD1308054
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2
  • PRKD2
  • Prkd2
  • LOC100407181
Reactivity
Human, Mouse (Murine)
120
29
18
5
5
1
1
Host
Rabbit
88
27
7
Clonality (Clone)
Monoclonal ()
Conjugate
This PKD2 antibody is un-conjugated
6
5
5
5
5
5
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
65
61
23
19
18
5
4
3
2
2
2
Options
Immunogen Recombinant protein within human Protein Kinase D2 aa 50-300
Clone 4C4
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others PKD2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PRKD2 (PKD2 Antibody Abstract)
Background

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

Subcellular location: Cytoplasm, Nucleus, Cell membrane, Golgi Apparatus


Synonyms: protein kinase D2, Serine/threonine-protein kinase D2, nPKC-D2, PRKD2, PKD2, HSPC187.
Gene ID 25865
UniProt Q9BZL6
Pathways cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity
Application Notes WB(1:1000-5000), FCM(1:50-100), IHC-P(1:50-200), IF(IHC-P)(1:50-200), IF(ICC)(1:50-100)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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