RAPGEF3 antibody (AA 700-900)

Catalog No. ABIN6944746

The Rabbit Monoclonal anti-RAPGEF3 antibody is suitable to detect RAPGEF3 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for RAPGEF3 antibody (AA 700-900) (ABIN6944746)

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAPGEF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3D1

Product Details anti-RAPGEF3 Antibody

Binding Specificity: AA 700-900

Purpose: RAPGEF3 (3D1) Monoclonal Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human Epac1 aa 700-900

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RAPGEF3

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Alternative Name: RAPGEF3

Background:

Synonyms: Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I, EPAC 1, cAMP-GEFI, RAPGEF3, CGEF1, EPAC, EPAC1, bcm910, HSU79275.

Background: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

Gene ID: 10411

UniProt: O95398

Pathways: cAMP Metabolic Process