RPA1 antibody

Catalog No. ABIN6944793

This Rabbit Monoclonal antibody specifically detects RPA1 in WB, FACS and IF (cc). It exhibits reactivity toward Human.

Quick Overview for RPA1 antibody (ABIN6944793)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 1C2

Product Details anti-RPA1 Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant protein corresponding to Human RPA70 aa 1-100

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1

Background:

Synonyms: Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein, RP-A p70, RF-A protein 1, RPA1, REPA1, RPA70

Background: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and coordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.

Gene ID: 6117

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA