Recombinant SET/TAF-I antibody (C-Term)

Catalog No. ABIN6944862

This Rabbit Monoclonal antibody specifically detects SET/TAF-I in WB, IF, FACS, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant SET/TAF-I antibody (C-Term) (ABIN6944862)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SET/TAF-I Antibody

Binding Specificity: C-Term

Purpose: SET Recombinant Antibody

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide within Human SET aa 200 to the C-terminus

Isotype: IgG

Application Details

Application Notes: WB(1:500-2000), IHC-P(1:100-200), IHC-F(1:100-200), IF(1:100-200), Flow-Cyt(1:50-100), ICC/IF(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET

Background:

Synonyms: Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, Template-activating factor I, IGAAD, I-2PP2A, TAF-I.

Background: Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins, however, isoform 2 specific activity is higher.

Gene ID: 6418

UniProt: Q01105

Pathways: Apoptosis