Recombinant Topoisomerase I antibody (AA 1-100)

Catalog No. ABIN6945193

The Rabbit Monoclonal anti-Topoisomerase I antibody is suitable to detect Topoisomerase I in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IHC (p), IF (cc), IHC (fro) and IF (p).

Quick Overview for Recombinant Topoisomerase I antibody (AA 1-100) (ABIN6945193)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Topoisomerase I Antibody

Binding Specificity: AA 1-100

Purpose: Topoisomerase I Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human Topoisomerase I between 1-100 amino acids

Isotype: IgG

Application Details

Application Notes: WB(WB(1:500-2000)), FCM(FCM(1:50-100)), IHC-P(IHC-P(1:50-200)), IHC-F(IHC-F(1:50-200)), IF(IHC-P)(IF(IHC-P)(1:50-200)), IF(ICC)(IF(ICC)(1:50-200))

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topoisomerase I

Background:

Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance