WT1 antibody (AA 50-250)

Catalog No. ABIN6945391

The Rabbit Monoclonal anti-WT1 antibody is suitable to detect WT1 in samples from Human and Mouse. It has been validated for IHC, WB, FACS, IHC (p) and IF (cc).

Quick Overview for WT1 antibody (AA 50-250) (ABIN6945391)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 9G6

Product Details anti-WT1 Antibody

Binding Specificity: AA 50-250

Purpose: Wilms Tumor Protein (9G6) Monoclonal Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Human Wilms Tumor Protein between 50 to 250 amino acids

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms Tumor Protein

Background:

Synonyms: Wilms tumor protein, WT33, WT1

Background: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation