Recombinant HIST1H4A antibody (H4K16ac)

Catalog No. ABIN6946913

The Rabbit Monoclonal anti-HIST1H4A antibody (ABIN6946913) specifically detects HIST1H4A in WB, FACS, IHC (p), IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant HIST1H4A antibody (H4K16ac) (ABIN6946913)

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HIST1H4A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-HIST1H4A Antibody

Binding Specificity: H4K16ac

Purpose: Histone H4 (acetyl K16) Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide corresponding to human histone H4 (acetyl K16).

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H4A

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Alternative Name: HIST1H4A

Background:

Synonyms: Histone H4, HIST1H4A, acetyl-Histone H4 (Lys16), H4K16Ac, histone cluster 4 H4, Histone H4 Acetylated on Lysine 16, H4 histone family

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins.

Gene ID: 121504

UniProt: P62805