BRD4 antibody (N-Term)

Catalog No. ABIN6952374

The Rabbit Polyclonal anti-BRD4 antibody has been validated for WB, ELISA, ChIP and ChIP-seq. It is suitable to detect BRD4 in samples from Human.

Quick Overview for BRD4 antibody (N-Term) (ABIN6952374)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-BRD4 Antibody

Binding Specificity: N-Term

Specificity: Polyclonal antibody raised in rabbit against human BRD4 (Bromodomain Containing 4), using two KLH-conjugated synthetic peptides from the N-terminal and central part of the protein, respectively.

Purification: Peptide affinity purified

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP 2 μg:ChIP
ELISA 1:10,000
WB 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.6 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: BRD4 (UniProt/Swiss-Prot entry O60885) is a chromatin reader protein that binds acetylated histones. It remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for gene transcription by preserving an acetylated chromatin status. As such, it plays a key role in the transmission of epigenetic memory across cell divisions. BRD4 promotes phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II and plays a key role in regulating the transcription of signal-inducible genes. It has been implicated in a translocation of chromosome 19 which causes an upper respiratory tract carcinoma.

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome