p300 antibody

Catalog No. ABIN6952378

The Mouse Monoclonal anti-p300 antibody (ABIN6952378) specifically detects p300 in ChIP and ChIP-seq. The antibody is reactive with Human samples.

Quick Overview for p300 antibody (ABIN6952378)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Grade: ChIP-seq Grade

Product Details anti-p300 Antibody

Specificity: Monoclonal antibody raised in mouse against human p300 (E1A Binding Protein P300) by DNA immunization in which the C-terminal part of the protein was cloned and expressed.

Purification: Protein A purified

Immunogen: DNA immunization

Isotype: IgG3

Application Details

Application Notes: ChIP/ChIP-seq 5 μg:ChIP

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300

Background: P300 (UniProt/Swiss-Prot entry Q09472) is a histone acetyltransferase that regulates transcription via chromatin remodelling. As such it is important for cell proliferation and differentiation. p300 is able to acetylate all four core histones in nucleosomes. Acetylation of histones is associated with transcriptional activation. p300 also acetylates non-histone proteins such as HDAC1 leading to its inactivation and modulation of transcription. It has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in the p300 gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size