H2AFZ antibody (acLys4)

Catalog No. ABIN6952410

The Rabbit Monoclonal anti-H2AFZ antibody has been validated for WB, IF, ChIP and ChIP-seq. It is suitable to detect H2AFZ in samples from Human.

Quick Overview for H2AFZ antibody (acLys4) (ABIN6952410)

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFZ antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-H2AFZ Antibody

Binding Specificity: acLys4

Specificity: Monoclonal antibody raised in rabbit against histone H2A.Z acetylated at Lys4 (H2A.ZK4ac), using a KLH-conjugated synthetic peptide.

Purification: Protein A purified

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP 0.5-1 μg:ChIP
WB 1:1,000
IF 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: PBS, 50 % glycerol, 1 % BSA , 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for H2AFZ

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name: H2A.Z

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of H2A.ZK4 is associated with gene activation.

UniProt: P0C0S5

Pathways: Telomere Maintenance