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Recombinant Blue Fluorescent Protein antibody (Alexa Fluor 647)

BFP Reactivity: Entacmaea quadricolor FACS, ICC, IF Host: Alpaca Monoclonal 1H7 Alexa Fluor 647 single-domain Antibody (sdAb) Recombinant Antibody
Catalog No. ABIN6953244
  • Target See all Blue Fluorescent Protein (BFP) products
    Blue Fluorescent Protein (BFP)
    Antibody Type
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reactivity
    Entacmaea quadricolor
    Host
    • 5
    Alpaca
    Expression System
    E.coli
    Clonality
    • 5
    Monoclonal
    Conjugate
    • 2
    • 2
    • 1
    This Blue Fluorescent Protein antibody is conjugated to Alexa Fluor 647
    Application
    Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Purpose
    Camelid sdAb anti-TagBFP conjugated with Alexa647, Clone 1H7
    Specificity
    Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
    Cross-Reactivity (Details)
    Does not cross-react with common GFP- or dsRed derivatives.
    Characteristics
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    Purification
    Produced in: E.coli
    Clone
    1H7
    Labeling Ratio
    Two site-specifically conjugated fluorophores per sdAb.
  • Application Notes
    Recommended dilution 1:500
    Comment

    Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    5 μM
    Buffer
    2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light!
    Storage
    -20 °C,-80 °C
    Storage Comment
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    Expiry Date
    12 months
  • Target
    Blue Fluorescent Protein (BFP)
    Alternative Name
    TagBFP (BFP Products)
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