CSF1R antibody (APC)

Catalog No. ABIN7278005

The Rat Monoclonal anti-CSF1R antibody has been validated for FACS. It is suitable to detect CSF1R in samples from Mouse.

Quick Overview for CSF1R antibody (APC) (ABIN7278005)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CSF1R antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: AFS98

Product Details anti-CSF1R Antibody (APC)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CD115 (c-fms)

Background: The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid pre- cursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteo- clasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor.

Gene ID: 12978

UniProt: P09581

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization