IL-12/IL-23 p40 antibody

Catalog No. ABIN6961468

This anti-IL-12/IL-23 p40 antibody is a Rat Monoclonal antibody detecting IL-12/IL-23 p40 in FACS, ELISA, IF, IP, Func and WB. Suitable for Mouse.

Quick Overview for IL-12/IL-23 p40 antibody (ABIN6961468)

Target: IL-12/IL-23 p40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-12/IL-23 p40 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func), Western Blotting (WB)

Clone: C17-8

Product Details anti-IL-12/IL-23 p40 Antibody

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Endotoxin Level: Low endotoxin: < 0.01 EU/ug

Isotype: IgG2a kappa

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Preservative: Azide free

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for IL-12/IL-23 p40

Target: IL-12/IL-23 p40

Background: The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein, known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, mac- rophages, and dendritic cells, and has been shown to target naive, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19 - its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the re- ceptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine.

Gene ID: 16160

UniProt: P43432