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IL-12/IL-23 p40 antibody

Reactivity: Mouse FACS, ELISA, IF, IP, WB, Func Host: Rat Monoclonal C17-8 unconjugated
Catalog No. ABIN6961468
  • Target See all IL-12/IL-23 p40 products
    IL-12/IL-23 p40
    Reactivity
    • 11
    • 3
    • 1
    Mouse
    Host
    • 11
    • 3
    Rat
    Clonality
    • 14
    Monoclonal
    Conjugate
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-12/IL-23 p40 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)
    Purification
    This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.
    Endotoxin Level
    Low endotoxin: < 0.01 EU/ug
    Clone
    C17-8
    Isotype
    IgG2a kappa
  • Application Notes
    This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.
    Comment

    2 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, pH 7.2
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    2-8°C
    Expiry Date
    12 months
  • Target
    IL-12/IL-23 p40
    Abstract
    IL-12/IL-23 p40 Products
    Background
    The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein, known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, mac- rophages, and dendritic cells, and has been shown to target naive, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19 - its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the re- ceptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine.
    Gene ID
    16160
    UniProt
    P43432
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