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CD3 epsilon antibody (PE)

CD3E Reactivity: Mouse FACS Host: Armenian Hamster Monoclonal 145-2C11 PE
Catalog No. ABIN7278261
  • Target See all CD3 epsilon (CD3E) Antibodies
    CD3 epsilon (CD3E)
    Reactivity
    • 216
    • 153
    • 51
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    Mouse
    Host
    • 133
    • 112
    • 33
    • 23
    • 19
    • 8
    • 1
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    Armenian Hamster
    Clonality
    • 210
    • 115
    • 6
    Monoclonal
    Conjugate
    • 122
    • 31
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    This CD3 epsilon antibody is conjugated to PE
    Application
    • 236
    • 135
    • 95
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    Flow Cytometry (FACS)
    Purification
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    Clone
    145-2C11
    Isotype
    IgG
    Top Product
    Discover our top product CD3E Primary Antibody
  • Application Notes
    This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
    Comment

    0.2 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    2-8°C protected from light
    Expiry Date
    12 months
  • Target
    CD3 epsilon (CD3E)
    Alternative Name
    CD3e (CD3E Products)
    Synonyms
    cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
    Background
    The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to rec- ognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activa- tion) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).
    Gene ID
    12501
    UniProt
    P22646
    Pathways
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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