Fc epsilon RI/FCER1A antibody (PE)

Catalog No. ABIN7278349

This Armenian Hamster Monoclonal antibody specifically detects Fc epsilon RI/FCER1A in FACS. It exhibits reactivity toward Mouse.

Quick Overview for Fc epsilon RI/FCER1A antibody (PE) (ABIN7278349)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This Fc epsilon RI/FCER1A antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MAR-1

Product Details anti-Fc epsilon RI/FCER1A Antibody (PE)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.

Comment:

0.2 mg/mL MC/9 (mouse mast cell line) cells were stained with 0.06 ug PE

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Alternative Name: FceR1

Background: The MAR-1 antibody reacts with the Fc epsilon Receptor I alpha chain (FceRIa), a transmembrane protein member of the Ig superfam- ily. This chain, together with a beta chain and two gamma chains form a tetrameric complex that supports IgE-mediated signaling and subsequent release of chemical mediators of allergy and immediate hypersensitivity. FceR1a is upregulated in the presence of IgE on those cell types which express it, such as Mast cells and Basophils. The MAR-1 antibody is widely used both in flow cytometry and for depletion of cells in vitro / in vivo.

Gene ID: 2205

UniProt: P20489

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process