IL-4 antibody (PE)

Catalog No. ABIN7278358

The Rat Monoclonal anti-IL-4 antibody has been validated for FACS. It is suitable to detect IL-4 in samples from Human.

Quick Overview for IL-4 antibody (PE) (ABIN7278358)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MP4-25D2

Product Details anti-IL-4 Antibody (PE)

Cross-Reactivity: Rhesus Monkey

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Comment:

5 uL (0.125 ug)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide. 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: The MP4-25D2 antibody is specific for human IL-4, a pleiotropic cytokine that Th2 cell differentiation, B cell proliferation and differentiation, and B cell class switching to IgE. MP4-25D2 is a neutralizing antibody and is reported to cross react with rhesus monkey IL-4.

Gene ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation