CD2 antibody (violetFluor™ 450)

Catalog No. ABIN7278531

This anti-CD2 antibody is a Mouse Monoclonal antibody detecting CD2 in FACS. Suitable for Human.

Quick Overview for CD2 antibody (violetFluor™ 450) (ABIN7278531)

Target: CD2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Clone: RPA-2-10

Product Details anti-CD2 Antibody (violetFluor™ 450)

Cross-Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Macaca nemestrina, Pig, Rhesus Monkey

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Comment:

5 μL (0.06 μg)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90 % of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.RPA-2.10 is reported to block mixed lymphocyte reaction. Please choose the appropriate format for each application.

Gene ID: 914

UniProt: Q6S161