CD44 antibody (violetFluor™ 500)

Catalog No. ABIN7278626

The Rat Monoclonal anti-CD44 antibody has been validated for FACS. It is suitable to detect CD44 in samples from Human and Mouse.

Quick Overview for CD44 antibody (violetFluor™ 500) (ABIN7278626)

Target: CD44

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is conjugated to violetFluor™ 500

Application: Flow Cytometry (FACS)

Clone: IM7

Product Details anti-CD44 Antibody (violetFluor™ 500)

Cross-Reactivity: Baboon, Cat, Chimpanzee, Cynomolgus, Dog, Horse, Pig, Rhesus Monkey

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500, eFluor® 506 or Pacific Orange®.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or "standard" form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, through which it mediates adhesion.The IM7 antibody may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain. (Xu et al, 2002, J. Leukoc. Biol. 72:1133-1141). It has been reported to be cross-reactive with many non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus, Horse, Cow, Pig, Dog and Cat CD44.

Gene ID: 12505, 960

UniProt: P16070, P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling