Interferon gamma antibody (PromoFluor 647 Premium)

Catalog No. ABIN6963771

The Mouse Monoclonal anti-Interferon gamma antibody is suitable to detect Interferon gamma in samples from Horse. It has been validated for FACS.

Quick Overview for Interferon gamma antibody (PromoFluor 647 Premium) (ABIN6963771)

Target: Interferon gamma (IFNG)

Reactivity: Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT166

Product Details anti-Interferon gamma Antibody (PromoFluor 647 Premium)

Specificity: Native and recombinant IFN-γ

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant equine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Detection of equine IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm. Detection of IFN-γ by flow cytometry in viable equine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT166- PF647P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 100034181

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy