IL-4 antibody (Biotin)

Catalog No. ABIN6963773

This Mouse Monoclonal antibody specifically detects IL-4 in ELISA and ELISpot. It exhibits reactivity toward Cow and Sheep and has been mentioned in 2+ publications.

Quick Overview for IL-4 antibody (Biotin) (ABIN6963773)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: BIL4-II

Product Details anti-IL-4 Antibody (Biotin)

Specificity: Native and recombinant IL-4

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-4 from sheep.

Characteristics: This monoclonal antibody enables specific detection of bovine and sheep IL-4 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant bovine IL-4

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963772 and ABIN7448241I is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963772 and ABIN7448241 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-4 antibody (Biotin) (ABIN6963773)

Rainard, Cunha, Gilbert: "Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland." in: PLoS ONE, Vol. 11, Issue 4, pp. e0154172, (2017) (PubMed).

Baquero, Plattner: "Bovine peripheral blood WC1+ and WC1neg γδ T lymphocytes modulate monocyte-derived macrophage effector functions during in vitro Mycobacterium avium subspecies paratuberculosis infection." in: Cellular immunology, Vol. 315, pp. 34-44, (2017) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Gene ID: 280824, 443327

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation