IL-5 antibody

Catalog No. ABIN6963823

This anti-IL-5 antibody is a Rat Monoclonal antibody detecting IL-5 in ELISA and ELISpot. Suitable for Mouse. This Primary Antibody has been cited in 4+ publications.

Quick Overview for IL-5 antibody (ABIN6963823)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: ELISA, ELISpot

Clone: TRFK5

Product Details anti-IL-5 Antibody

Specificity: Native and recombinant IL-5

Characteristics: This monoclonal antibody enables specific detection of mouse IL-5 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse IL-5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963823, ABIN7448276, ABIN6963894, ABIN7489155, ABIN6964015 and ABIN6964027 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963824 and ABIN7448277 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-5 antibody (ABIN6963823)

Wang, Chen, Smith, Cao, Acosta, Fan, Ciotti, Fattom, Baker: "A novel combination of intramuscular vaccine adjuvants, nanoemulsion and CpG produces an effective immune response against influenza A virus." in: Vaccine, Vol. 38, Issue 19, pp. 3537-3544, (2020) (PubMed).

Sakurai, Inamine, Iinuma, Funakoshi, Yonekura, Sakurai, Hanazawa, Nakayama, Ishii, Okamoto: "Activation of invariant natural killer T cells in regional lymph nodes as new antigen-specific immunotherapy via induction of interleukin-21 and interferon-γ." in: Clinical and experimental immunology, Vol. 178, Issue 1, pp. 65-74, (2014) (PubMed).

Inamine, Sakurai, Horiguchi, Yonekura, Hanazawa, Hosokawa, Matuura-Suzuki, Nakayama, Okamoto: "Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells." in: Clinical immunology (Orlando, Fla.), Vol. 143, Issue 2, pp. 170-9, (2012) (PubMed).

Lipscomb, Chen, Taylor, Goldbach, Watkins, Kalinski, Butterfield, Wesa, Storkus: "Ectopic T-bet expression licenses dendritic cells for IL-12-independent priming of type 1 T cells in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7250-8, (2009) (PubMed).

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Gene ID: 16191

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour