IL-4 antibody (Biotin)

Catalog No. ABIN6963826

This anti-IL-4 antibody is a Mouse Monoclonal antibody detecting IL-4 in ELISA and ELISpot. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 5+ publications.

Quick Overview for IL-4 antibody (Biotin) (ABIN6963826)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: IL4-II

Product Details anti-IL-4 Antibody (Biotin)

Specificity: Native and recombinant IL-4

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-4 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-4 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963825 and ABIN7448278I is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963825 and ABIN7448278 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-4 antibody (Biotin) (ABIN6963826)

Lambracht-Washington, Fu, Frost, Rosenberg: "Evaluation of a DNA Aβ42 vaccine in adult rhesus monkeys (Macaca mulatta): antibody kinetics and immune profile after intradermal immunization with full-length DNA Aβ42 trimer." in: Alzheimer's research & therapy, Vol. 9, Issue 1, pp. 30, (2018) (PubMed).

Holbrook, Kim, Blevins, Jorgensen, Kock, DAgostino, Aycock, Hadimani, King, Parks, Alexander-Miller: "A Novel R848-Conjugated Inactivated Influenza Virus Vaccine Is Efficacious and Safe in a Neonate Nonhuman Primate Model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 197, Issue 2, pp. 555-64, (2017) (PubMed).

Camarca, Gianfrani, Ariemma, Cimmino, Bruzzese, Scerbo, Picascia, DEsposito, Beguinot, Formisano, Valentino: "Human Peripheral Blood Mononuclear Cell Function and Dendritic Cell Differentiation Are Affected by Bisphenol-A Exposure." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161122, (2017) (PubMed).

Riccio, Pratt-Riccio, Bianco-Júnior, Sanchez, Totino, Carvalho, Daniel-Ribeiro: "Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research." in: Malaria journal, Vol. 14, pp. 166, (2016) (PubMed).

Shastri, Stewart, Eapen, Peterson, Zaidi, Gueven, Sohal, Patel: "Opposing effects of low molecular weight heparins on the release of inflammatory cytokines from peripheral blood mononuclear cells of asthmatics." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118798, (2015) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Gene ID: 3565

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation