IL-4 antibody (PromoFluor 647 Premium)

Catalog No. ABIN6963828

The Rat Monoclonal anti-IL-4 antibody has been validated for FACS. It is suitable to detect IL-4 in samples from Human and Non-Human Primate.

Quick Overview for IL-4 antibody (PromoFluor 647 Premium) (ABIN6963828)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: IL4-3

Product Details anti-IL-4 Antibody (PromoFluor 647 Premium)

Specificity: Native IL-4

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-4 from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-4

Isotype: IgG1

Application Details

Application Notes: Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF647P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Gene ID: 3565

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation